ABSTRACT
Introduction: The moral stressor situations is considered one of the issues and problems to professional nurses that can be affected negatively Their function and quality of care, the goal of present study is determining the amount and abundance of moral distress in clinical nurses and related factors in city of Shahroud
Methods: In this Cross-sectional study; 261 nurses that worked in Imam Hussein and Fatemiyeh hospitals of shahroud were studied in 2013-2014. The instrument of collecting data was consisted of demographic and occupational form and Corley moral distress questionnaire. Data were analyzed with using descriptive statics and Pearson correlation coefficient, independent T test and one-way ANOVA statistical tests
Results: The frequency and severity of moral distress in most of nurses was in the medium level and there was observed significant statistically difference between frequency and severity of moral distress in nurses [p<0.001].In the aspect the frequency, emergency and psychiatric nurses and about intensity, nurses working in departments of emergency, orthopedic and ICU reported the highest score of moral distress. There was a significant negative correlation between the age [p<0.001], job experience [p=0.01] and total score of moral distress and There was a significant difference between the type of employment and the moral distress [p<0.05] but there was no significant association between sexuality, marital status and monthly working hours with moral distress [p>0.05]
Conclusion: Considering the prevalence of moral distress among nurses, presenting strategies to reduce according to the terms with any parts is necessary. Provide the necessary training and use of nurses with age more work experience in order to how to cope with moral tension is recommended
ABSTRACT
Background: Purity of cultured Schwann cell is very important in patients' outcome. The purpose of this study was evaluation of cell purity in a culture without nerve growth factors and fetal bovine serum
Materials and Methods: In this experimental study, for culture of human Schwann cells, nerve growth factors and fetal bovine serum were replaced by human autologous serum. Obtaining a consent from the close relative, nerve grafts were harvested and transported to processing unit where they were cultured in DMEM upon aseptic condition. Then the cultured cells were evaluated with S100 antibody staining for both morphology and purity
Results: Cell purity range was from 97% to 99% [mean=98.11+/-0.782%]. Cell count was 14055.56+/-2480.479 per microliter. There was not significant correlation between cell purity with either the culture period or the age of donors [P>0.05]. The Spearman correlation coefficient for the cell purity with the culture period and the age of donors was -0.21 and -0.09, respectively
Conclusion: We found that the replacement of nerve growth factors and fetal bovine serum with human autologous serum improves the cultured Schwann cells for clinical use with more safety and minimum reagents